Comparative Analysis of Protein Profile of Vaccine Strain and Local Isolates of Avian Infectious Bursal Disease Virus by Western Blot
نویسنده
چکیده
Infectious bursal disease (IBD), commonly known as “Gumboro Disease is an infectious viral disease of chickens inducing severe immunosuppression. In Pakistan, imported vaccine is used to prevent the disease but there have been outbreaks even in vaccinated flocks. This indicates that there is variation among the locally prevalent viral strains and commercially available vaccine strain. In present study, twenty-five local strains of IBD virus (IBDV) were isolated, purified and their structural polypeptides were studied by polyacrylamide gel electrophoresis (PAGE). Then their protein profiles were compared with commercial vaccine D-78 strain. Distinct banding patterns were observed in local isolates on basis of which the viral isolates were placed in three groups. Group-1 contained polypeptides of molecular weights 95Kd, 60Kd, 56Kd, 45Kd, 31Kd, 21Kd and 14Kd. In group-2, bands of 56Kd and 32Kd were missing whereas in group3 the bands of 56Kd and 31Kd were missing while comparing with group-1. It was observed that 60Kd molecular weight protein band was common in all the three groups but it was absent in the vaccine D-78 strain. Vaccine D-78 strain appeared to have the banding pattern as 95Kd, 53Kd, 46Kd, 40Kd, 32Kd and 27Kd. Western blot analysis of the proteins were also conducted using antibodies raised against vaccine strain D-78. It was observed that the antibodies bind against all the protein bands of vaccine D-78 but did not bind with all the polypeptides of locally prevalent field viruses. This confirms that antigenic heterogenicity exists among the indigenous strains and the imported vaccine strains of IBDV.
منابع مشابه
Effect of experimental infectious bursal disease virus on clinical signs and pathogenesis of avian influenza virus H9N2 in turkey by real time PCR
Infectious bursal disease virus (IBDV) in turkeys may result in immunosuppression, and inability of turkeys to resist nonpathogenic or less pathogenic organisms. A total number of 120 day-old commercial male turkeys were purchased and blood samples were collected from 20 day-old turkeys, remaining 100 were divided into four equal groups and kept in separated rooms. Groups 1 and 2 were infected ...
متن کاملCloning and Codon-optimized Expression of Structural Protein Hypervariable Region of VP2 from Infectious Bursal Disease Virus
Infectious bursal disease virus (IBDV) is the causative agent of Gumboro disease, an infectious disease of global economic importance in poultry. Structural protein VP2 of IBDV is the most frequently studied protein due to its significant roles in virus attachment, protective immunity, and serotype specificity. The objective of the present study was to improve the expression of hypervariable re...
متن کاملCloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells
VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was clonedin a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leadersequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Theconstruct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of...
متن کاملEvaluation of Experimental Inactivated Infectious Bursal Disease Vaccine Using a Local Very Virulent Pathotype Strain
متن کامل
Sequence analysis of the VP1 gene in three very virulent Iranian infectious bursal disease virus strains
Infectious bursal disease (IBD) is a highly contagious disease of chickens caused by the infectious bursal disease virus (IBDV). This study was conducted to characterize three IBDV strains from Iran. A reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was used to amplify a 715-bp fragment of the VP1 gene from IBDV strains. Amplified VP1 fragments of the three Iranian IBDV strai...
متن کامل